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fitc mouse anti mouse cd45 1  (Thermo Fisher)


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    Thermo Fisher fitc mouse anti mouse cd45 1
    Fitc Mouse Anti Mouse Cd45 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc mouse anti mouse cd45 1/product/Thermo Fisher
    Average 94 stars, based on 49 article reviews
    fitc mouse anti mouse cd45 1 - by Bioz Stars, 2026-02
    94/100 stars

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    Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse <t>CD326</t> (EPCAM) (green) and Alex594-conjugated antibodies against <t>CD45</t> (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.
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    Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse <t>CD326</t> (EPCAM) (green) and Alex594-conjugated antibodies against <t>CD45</t> (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.
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    Image Search Results


    Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

    Journal: iScience

    Article Title: Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis.

    doi: 10.1016/j.isci.2024.109547

    Figure Lengend Snippet: Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

    Article Snippet: Antibodies Anti-pan cytokeratin Abcam ab7753 P-Selectin polyclonal antibody Proteintech 13304-1-AP CD45R (RA3-6B2) Santa Cruz sc-19597 Alexa Fluor 488-labeled goat anti-mouse lgG (H + L) Proteintech SA00013-1 594-labeled goat anti-rabbit lgG (H + L) Proteintech SA00013-4 TRITIC-labeled goat anti-Rat lgG (H + L) Proteintech SA00007-7 CD45 Rabbit Polyclonal antibody Proteintech 20103-1-AP FITC anti-mouse CD326 (Ep-CAM) Biolegend 118207 Alex 594 anti-mouse CD45 Biolegend 103144 Chemicals, peptides, and recombinant proteins Fetal bovine serum ExCell Bio FSP500 RPMI-1640 medium HyClone SH30027.02

    Techniques: Activation Assay, Control, Staining, Gene Expression, Cell Culture